Emerging Concepts in Neuro-Oncology by Colin Watts

Emerging Concepts in Neuro-Oncology by Colin Watts

Author:Colin Watts
Language: eng
Format: epub
Publisher: Springer London, London


Cell Irradiation and Particle Beams

Cell irradiation experiments are the cornerstone of classical radiation biology. The most common technique that is used is the clonogenic survival assay. In this technique, the surviving fraction of cells exposed to varying doses of radiation is established by irradiating plates of cells at low density and subsequently counting the number of viable colonies per unit area. The surviving fraction is expressed relative to the colony formation of a control plate which is not irradiated. The typical clonogenic survival curve is plotted with the survival fraction on a log scale on the y-axis and the radiation dose on a linear scale on the x-axis (Fig. 7.4).

Fig. 7.4Clonogenic survival curve for T98G human glioma cell line for x-ray irradiation



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